Journal article
Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenetic protein 15 and follicle stimulating hormone
ML Sutton-Mcdowall, M Purdey, HM Brown, AD Abell, DG Mottershead, PD Cetica, GC Dalvit, EM Goldys, RB Gilchrist, DK Gardner, JG Thompson
Molecular Reproduction and Development | Published : 2015
DOI: 10.1002/mrd.22470
Abstract
The developmental competence of cumulus oocyte complexes (COCs) can be increased during in vitro oocyte maturation with the addition of exogenous oocyte-secreted factors, such as bone morphogenetic protein 15 (BMP15), in combination with hormones. FSH and BMP15, for example, induce different metabolic profiles within COCs-namely, FSH increases glycolysis while BMP15 stimulates FAD and NAD(P)H accumulation within oocytes, without changing the redox ratio. The aim of this study was to investigate if this BMP15-induced NAD(P)H increase was due to de novo NADPH production. Cattle COCs were cultured with FSH and/or recombinant human BMP15, resulting in a significant decrease in glucose-6-phosphat..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
Grant sponsor: National Health and Medical Research Council Australia (NHMRC) Project; Grant number: 1008137; Grant sponsor: NHMRC Development; Grant number: 1017484; Grant sponsor: NHMRC Fellowship; Grant number: 627007; Grant sponsor: Australian Research Council Centre of Excellence for Nanoscale BioPhotonics; Grant number: CE140100003